Viability of Postmortal Epididymal Mouse Spermatozoa during Long-Term Hypothermic Storage and Cryopreservation

被引：0
|
作者：
N. V. Shishova
E. N. Gakhova
E. V. Mel’nikova
机构：
[1] Russian Academy of Sciences,Laboratory of Genetic Resources Cryoconservation, Institute of Cell Biophysics
来源：
Bulletin of Experimental Biology and Medicine | 2011年 / 151卷
关键词：
cryoconservation; hypothermia; postmortal epididymal spermatozoa; laboratory mouse; capacitation;
D O I：
暂无
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学科分类号：
摘要：
The study examined the effect of long-term hypothermic (4°C) storage of mouse carcasses on motility, cell membrane damage, in vitro survival and capacitation of epididymal spermatozoa before and after cryopreservation. It was shown that the number of spermatozoa with rectilinear forward motion decreased with increasing storage time. There were no significant changes in the total sperm motility and integrity of their plasmalemma. Pronounced effects of hypothermia and long-term storage of the mouse carcasses on cryocapacitation of spermatozoa during cryoconservation were demonstrated.
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页码：440 / 444
页数：4
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