Effect of genotype, medium and sugar on triticale (× Triticosecale Wittm.) anther culture response.

The response of sixteen triticale genotypes to the anther culture induction medium composition was investigated. Two sugar versions of P2 and C17 media were tested, containing 9 % of either maltose or sucrose. Subsequently obtained embryo-like structures were regenerated on 190-2 medium. The results showed the significant differences between genotypes for all the investigated traits. Green plants were obtained at frequency ranging from 0 to 2.3 per 100 plated anthers. Maltose was superior to sucrose (on average respectively 0.69 and 0.03 green plants per 100 plated anthers). P2 with maltose yielded the highest number of green plants in more genotypes than either of remaining media. The genotype × medium, genotype × sugar and medium × sugar interaction appeared significant. Both, P2 and CI7 media supplemented with maltose are advisable for triticale haploid production.