Kinetic studies on the oxidation of cytochrome b5 Phe35 mutants with cytochrome c, plastocyanin and inorganic complexes

被引：0
|
作者：
Ping Yao
Yun-Hua Wang
Bing-Yun Sun
Yi Xie
Shun Hirota
Osamu Yamauchi
Zhong-Xian Huang
机构：
[1] Chemical Biology Laboratory,
[2] Department of Chemistry,undefined
[3] Fudan University,undefined
[4] Shanghai 200433,undefined
[5] State Key Laboratory of Genetics,undefined
[6] School of Life Science,undefined
[7] Fudan University,undefined
[8] Shanghai 200433,undefined
[9] Department of Chemistry,undefined
[10] School of Science and Research Center for Material Science,undefined
[11] Nagoya University,undefined
[12] Chikusa-ku,undefined
[13] Nagoya 464-8602,undefined
来源：
JBIC Journal of Biological Inorganic Chemistry | 2002年 / 7卷
关键词：
Mutation Cytochrome b5 Plastocyanin Cytochrome c Electron transfer;
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学科分类号：
摘要：
To illustrate the functions of the aromatic residue Phe35 of cytochrome b5 and to give further insight into the roles of the Phe35-containing hydrophobic patch and/or aromatic channel of cytochrome b5, we studied electron transfer reactions of cytochrome b5 and its Phe35Tyr and Phe35Leu variants with cytochrome c, with the wild-type and Tyr83Phe and Tyr83Leu variants of plastocyanin, and with the inorganic complexes [Fe(EDTA)]–, [Fe(CDTA)]– and [Ru(NH3)6]3+. The changes at Phe35 of cytochrome b5 and Tyr83 of plastocyanin do not affect the second-order rate constants for the electron transfer reactions. These results show that the invariant aromatic residues and aromatic patch/channel are not essential for electron transfer in these systems.
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页码：375 / 383
页数：8
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