Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

被引：0

作者：

Alexis C. Komor

Yongjoo B. Kim

Michael S. Packer

John A. Zuris

David R. Liu

机构：

[1] Harvard University,Department of Chemistry and Chemical Biology

[2] Howard Hughes Medical Institute,undefined

[3] Harvard University,undefined

来源：

Nature | 2016年 / 533卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

CRISPR/Cas9 DNA editing creates a double-stranded break in the target DNA, which can frequently generate random insertion or deletion of bases (indels); a new genome editing approach combining Cas9 with a cytidine deaminase is described here, which corrects point mutations more efficiently than canonical Cas9, while avoiding double-stranded breaks and indel formation.

引用

页码：420 / 424

页数：4

共 50 条

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NATURE, 2016, 533 (7603) : 420 - +
[2] Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
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