Study on the classification and identification of microorganisms in municipal sludge

被引:0
|
作者
Kegui Zhang
Xiaomei Zhang
Qianqian Yang
机构
[1] Huainan Normal University,School of Biological Engineering
来源
Applied Nanoscience | 2023年 / 13卷
关键词
Municipal sludge; Microbial classification and identification; PCR technology; Support vector machine;
D O I
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中图分类号
学科分类号
摘要
To solve the problem that the traditional microbial classification and identification methods cannot output the corresponding results in time when the number of microorganisms to be identified changes, this paper studies the classification and identification methods of microorganisms in urban domestic sludge. The microorganisms in municipal sewage sludge are collected by sedimentation, and the classification standard of microorganisms in municipal sewage sludge is established. Extract the DNA sequence of microorganisms and analyze the information in the DNA sequence by PCR. Collect protozoan images in the sludge, and extract image features after pre-processing. Using DNA information characteristics and protozoan image characteristics as the basis for classification and identification, and using support vector machines to complete the classification and identification of microorganisms in urban domestic sludge, the design of methods for classification and identification of microorganisms was realized. Through comparison experiments with traditional classification and identification methods, it is proved that the studied microbial classification and identification methods can quickly respond when the number of microorganisms in the identification target changes.
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页码:2115 / 2128
页数:13
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