Rapid detection of a minute amount of lipopolysaccharide from Salmonella in large volumes by poly(ethyleneimine)-cloth enzyme immunoassay

被引：0
|
作者：
Kouchi Matsumoto
Masayo Sakata
Masashi Kunitake
Masao Miyazakia
Chuichi Hirayama
机构：
[1] Kumamoto University,Department of Applied Chemistry & Biochemistry, Faculty of Engineering
[2] Ricoh Kyosan,undefined
[3] Inc.,undefined
来源：
Biotechnology Letters | 2002年 / 24卷
关键词：
cloth enzyme immunoassay; lipopolysaccharide; poly(ethylene imine);
D O I：
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中图分类号：
学科分类号：
摘要：
Utilizing the rapidity in capturing Salmonella lipopolysaccharide (LPS) and the macroporous nature of poly(ethyleneimine) immobilized polyester cloth (PEI-cloth), a PEI-cloth disk set in a Swinnex cartridge joined at both ends with 10 ml plastic syringes was repeatedly passed through with an LPS sample in large volumes. The captured LPS on the disk were detected at up to 1 pg, which is equivalent to the amount produced of 16 cells, by cloth enzyme immunoassay.
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页码：1791 / 1795
页数：4
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