The enhancement of osteogenesis by scaffold based on mineralized recombinant human-like collagen loading with rhBMP-2

被引：0
|
作者：
Bin Wu
Qixin Zheng
Xiaodong Guo
Yongchao Wu
Yu Wang
Fuzai Cui
机构：
[1] Huazhong University of Science & Technology,Department of Orthopaedics, Union Hospital, Tongji Medical College
[2] Tsinghua University,Biomaterials Laboratory, Department of Materials Science & Engineering
来源：
Journal of Wuhan University of Technology-Mater. Sci. Ed. | 2009年 / 24卷
关键词：
rhBMP-2; bone tissue engineering; osteogenesis; biomaterial;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A biomimetic scaffold based on mineralized recombinant collagen, nano-hydroxyapatite/recombinant human-like collagen/poly(lactic acid) (nHA/RHLC/PLA), was prepared with recombinant human bone morphogenic protein-2 (rhBMP-2) for improving the osteoinductive property of the scaffold. The nHA/RHLC/PLA scaffolds loaded with 10 µg rhBMP-2 and the unloaded scaffolds were implanted subcutaneously in the rat model. The osteogenetic capacity of these composites was evaluated by CT scan, ALP activity test and histological observation at 4 and 8 weeks after implantation. The experimental results indicated that the osteogenic capability of the scaffolds loaded with rhBMP-2 was superior to the unloaded scaffold. It was concluded that rhBMP-2 can enhance the osteoinductive property of the nHA/RHLC/PLA scaffold and the nHA/RHLC/PLA scaffold loaded with rhBMP-2 have the good potential of being used in bone tissue engineering.
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页码：956 / 960
页数：4
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