Optimization of extraction and purification of glucoamylase produced by Aspergillus awamori in solid-state fermentation

被引：0
|
作者：
Sangeeta Negi
Rintu Banerjee
机构：
[1] Motilal Nehru National Institute of Technology,Biotechnology Section, Applied Mechanics Department
[2] Indian Institute of Technology,Agriculture and food Engineering Department, Microbial Biotechnology and Downstream Processing Laboratory
来源：
Biotechnology and Bioprocess Engineering | 2009年 / 14卷
关键词：
amylase; enzyme extraction; gel filtration; SDS-PAGE;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Various parameters such as solvent selection, concentration, soaking time, and temperature were tested in a single bioreactor in order to determine optimum extraction conditions of glucoamylase, when produced simultaneously with protease by Aspergillus awamari nakazawa MTCC 6652. Optimum conditions were achieved in a 10% glycerol solution soaked for 2 h at 40°C, followed by concentration of extracted glucoamylase (9,157 U/gds) by acetone precipitation (1:2, v/v), which yielded 51.9% recovery. Ion exchange chromatography and gel filtration showed specific activities of 270.5 and 337.5 U/mg, respectively, while SDS-PAGE and zymogram analysis of glucoamylase indicated the presence of three starch-hydrolyzing isoforms with molecular weights of approximately 109.6, 87.1, and 59.4 kDa, respectively
引用
收藏
页码：60 / 66
页数：6
相关论文
共 50 条
  • [1] Optimization of extraction and purification of glucoamylase produced by Aspergillus awamori in solid-state fermentation
    Negi, Sangeeta
    Banerjee, Rintu
    [J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2009, 14 (01) : 60 - 66
  • [2] Extraction and Purification of Glucoamylase and Protease Produced by Aspergillus awamori in a Single-Stage Fermentation
    Negi, Sangeeta
    Gupta, Suneel
    Banerjee, Rintu
    [J]. FOOD TECHNOLOGY AND BIOTECHNOLOGY, 2011, 49 (03) : 310 - 315
  • [3] Purification and characterization of acid-stable protopectinase produced by Aspergillus awamori in solid-state fermentation
    Nagai, M
    Ozawa, A
    Katsuragi, T
    Sakai, T
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2000, 64 (07) : 1337 - 1344
  • [4] Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation
    Selvakumar, P
    Ashakumary, L
    Helen, A
    Pandey, A
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 1996, 23 (06) : 403 - 406
  • [5] Optimization of glucoamylase production by Aspergillus niger in solid-state fermentation
    Silveira, ST
    Oliveira, MS
    Costa, JAV
    Kalil, SJ
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2006, 128 (02) : 131 - 139
  • [6] Optimization of glucoamylase production by Aspergillus niger in solid-state fermentation
    Silvana T. Silveira
    Melissa S. Oliveira
    Jorge A. V. Costa
    Susana J. Kalil
    [J]. Applied Biochemistry and Biotechnology, 2006, 128 : 131 - 139
  • [7] Biochemical Characterisation of a Glucoamylase from Aspergillus niger Produced by Solid-State Fermentation
    Slivinski, Christiane Trevisan
    Lopes Machado, Alex Vinicius
    Iulek, Jorge
    Ayub, Ricardo Antnio
    de Almeida, Mareci Mendes
    [J]. BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, 2011, 54 (03) : 559 - 568
  • [8] Extraction and purification of amyloglucosidase produced by solid state fermentation with Aspergillus niger
    Ramadas, M
    Holst, O
    Mattiasson, B
    [J]. BIOTECHNOLOGY TECHNIQUES, 1995, 9 (12) : 901 - 906
  • [9] Reverse Micellar Extraction of Fungal Glucoamylase Produced in Solid-State Fermentation Culture
    Paraj, Aliakbar
    Khanahmadi, Morteza
    Karimi, Keikhosro
    Taherzadeh, Mohammad J.
    [J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 24 (12) : 1690 - 1698
  • [10] Polygalacturonase production by Aspergillus awamori on wheat in solid-state fermentation
    Blandino, A
    Iqbalsyah, T
    Pandiella, SS
    Cantero, D
    Webb, C
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 58 (02) : 164 - 169
12345