Genetically encoded releasable photo-cross-linking strategies for studying protein–protein interactions in living cells

被引:0
|
作者
Yi Yang
Haiping Song
Dan He
Shuai Zhang
Shizhong Dai
Xiao Xie
Shixian Lin
Ziyang Hao
Huangtao Zheng
Peng R Chen
机构
[1] Synthetic and Functional Biomolecules Center,
[2] Beijing National Laboratory for Molecular Sciences,undefined
[3] Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,undefined
[4] College of Chemistry and Molecular Engineering,undefined
[5] Peking University,undefined
[6] Peking-Tsinghua Center for Life Sciences,undefined
来源
Nature Protocols | 2017年 / 12卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
This protocol describes strategies for the characterization of transient protein–protein interactions and their interaction interfaces via genetically encoded releasable photo-cross-linkers.
引用
收藏
页码:2147 / 2168
页数:21
相关论文
共 50 条
  • [1] Genetically encoded releasable photo-cross-linking strategies for studying protein-protein interactions in living cells
    Yang, Yi
    Song, Haiping
    He, Dan
    Zhang, Shuai
    Dai, Shizhong
    Xie, Xiao
    Lin, Shixian
    Hao, Ziyang
    Zheng, Huangtao
    Chen, Peng R.
    NATURE PROTOCOLS, 2017, 12 (10) : 2147 - 2168
  • [2] A photo-cross-linking approach to monitor protein dynamics in living cells
    Miyazaki, Ryoji
    Akiyama, Yoshinori
    Mori, Hiroyuki
    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2020, 1864 (02):
  • [3] A Method for Protein Photo-cross-linking in Living Cells Facilitating Analysis of Physiological Interactions of Proteins
    Hino, Nobumasa
    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 2015, 135 (12): : 1357 - 1363
  • [4] Photo-cross-linking interacting proteins with a genetically encoded benzophenone
    Ian S Farrell
    Rebecca Toroney
    Jennifer L Hazen
    Ryan A Mehl
    Jason W Chin
    Nature Methods, 2005, 2 : 377 - 384
  • [5] Photo-cross-linking interacting proteins with a genetically encoded benzophenone
    Farrelli, IS
    Toroney, R
    Hazen, JL
    Mehl, RA
    Chin, JW
    NATURE METHODS, 2005, 2 (05) : 377 - 384
  • [6] A NEW PHOTO-CROSS-LINKING REAGENT FOR THE STUDY OF PROTEIN-PROTEIN INTERACTIONS
    RESEK, JF
    BHATTACHARYA, S
    KHORANA, HG
    JOURNAL OF ORGANIC CHEMISTRY, 1993, 58 (26): : 7598 - 7601
  • [7] A Photo-Cross-Linking Strategy to Map Sites of Protein-Protein Interactions
    Chen, Yihui
    Wu, Yalin
    Henklein, Peter
    Li, Xiaoxu
    Hofmann, Klaus Peter
    Nakanishi, Koji
    Ernst, Oliver P.
    CHEMISTRY-A EUROPEAN JOURNAL, 2010, 16 (25) : 7389 - 7394
  • [8] Genetically Encoded 2-Aryl-5-carboxytetrazoles for Site-Selective Protein Photo-Cross-Linking
    Tian, Yulin
    Jacinto, Marco Paolo
    Zeng, Yu
    Yu, Zhipeng
    Qu, Jun
    Liu, Wenshe R.
    Lin, Qing
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2017, 139 (17) : 6078 - 6081
  • [9] Genetically Encoded Residue-Selective Photo-Crosslinker to Capture Protein-Protein Interactions in Living Cells
    Hu, Wei
    Yuan, Yi
    Wang, Cai-Hu
    Tian, Hong-Tao
    Guo, An-Di
    Nie, Hui-Jun
    Hu, Hao
    Tan, Minjia
    Tang, Zhuo
    Chen, Xiao-Hua
    CHEM, 2019, 5 (11): : 2955 - 2968
  • [10] IDENTIFICATION OF THE TARGET OF A TRANSCRIPTION ACTIVATOR PROTEIN BY PROTEIN-PROTEIN PHOTO-CROSS-LINKING
    CHEN, Y
    EBRIGHT, YW
    EBRIGHT, RH
    SCIENCE, 1994, 265 (5168) : 90 - 92