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Simple Extraction and Rapid Quantitative Analysis of Isoflavones in Soybean Seeds

被引：0
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作者：
D. Heimler
P. Vignolini
C. Galardi
P. Pinelli
A. Romani
机构：
[1] University of Florence,Department of Soil Science and Plant Nutrition
[2] University of Florence,Department of Pharmaceutical Science
来源：
Chromatographia | 2004年 / 59卷
关键词：
Column liquid chromatography; Diode array and MS detection; Isoflavones; Soybean seeds; Flavonoids;
D O I：
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中图分类号：
学科分类号：
摘要：
A simple extraction and rapid HPLC method, suitable to separate and quantify the isoflavonoids in soybean seeds, are proposed and discussed. The method has been applied to the separation and quantification of seed isoflavonoids in five naturally grown soy cultivars; the total amount of isoflavones ranged from 1.83 g kg−1 to 11.88 g kg−1 of fresh weight. The detection limits (200 μg/kg−1), evaluated for genistein and daidzein, are presented together with a list of the soy polyphenols analysed by HPLC-DAD and HPLC-MS.
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页码：361 / 365
页数：4
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