Taurine transporter is expressed in vascular smooth muscle cells

被引：0
|
作者：
X.-B. Liao
X.-M. Zhou
J.-M. Li
Z.-P. Tan
L.-M. Liu
W. Zhang
H. Tan
Y. Lu
L.-Q. Yuan
机构：
[1] Central South University,Department of Cardiothoracic Surgery, The Second Xiang
[2] Central South University,Ya Hospital
来源：
Amino Acids | 2007年 / 33卷
关键词：
Keywords: Taurine transporter – Vascular smooth muscle cells – Kinetic analysis;
D O I：
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中图分类号：
学科分类号：
摘要：
The regulation of vascular smooth muscle cells (VSMCs) function by taurine has been a subject of increasing interest and investigation, and taurine is taken up into cells through a specific transporter system, the taurine transporter (TAUT). In the present study, we examined the expression of TAUT in VSMCs and the kinetic parameters of the uptake process of TAUT in VSMCs. RT-PCR and western blot demonstrated that the mRNA and protein of TAUT was expressed in VSMCs in vitro. Immunohistochemistry using antibody for TAUT revealed the expression of this protein in rat thoracic aorta. The maximal [3H]taurine uptake rate in VSMCs was 37.75 ± 3.13 pmol/min per mg of protein, with a Km value of 5.42 ± 0.81 µM. Thus, VSMCs are able to express a functional taurine transporter. The regulation and detailed function of taurine and TAUT in VSMCs remain unclear, but our findings suggest a functional role for them in VSMCs metabolism.
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页码：639 / 643
页数：4
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