Atmospheric bioaerosol, Bacillus sp., at an altitude of 3,500 m over the noto peninsula: Direct sampling via aircraft

被引：0

作者：

Kobayashi F. ^{[1
]}

Morosawa S. ^{[2
]}

Maki T. ^{[1
]}

Kakikawa M. ^{[3
]}

Yamada M. ^{[4
]}

Tobo Y. ^{[5
]}

Hon C.-S. ^{[6
]}

Matsuki A. ^{[5
]}

Iwasaka Y. ^{[5
]}

机构：

[1] College of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa

[2] Graduate School of Natural Sciences and Technology, Kanazawa University, Kakuma-machi, Kanazawa

[3] Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa

[4] Center for Innovation, Kanazawa University, Kakuma-machi, Kanazawa

[5] Frontier Science Organization, Kanazawa University, Kakuma-machi, Kanazawa

[6] College of Medical, Pharmaceutical and Health Science, Kanazawa University, Kakuma-machi, Kanazawa

来源：

Asian Journal of Atmospheric Environment | 2011年 / 5卷 / 3期

关键词：

Aircraft; Atmospheric bioaerosols; Bacillus sp; Direct sampling; Noto peninsula;

D O I：

10.5572/ajae.2011.5.3.164

中图分类号：

学科分类号：

摘要：

This work focuses on the analysis of bioaerosols in the atmosphere at higher altitudes over Noto Peninsula, Japan. We carried out direct sampling via aircraft, separated cultures, and identified present isolates. Atmospheric bioaerosols at higher altitudes were collected using a Cessna 404 aircraft for an hour at an altitude of 3,500 m over the Noto Peninsula. The aircraft-based direct sampling system was devised to improve upon the system of balloon-based sampling. In order to examine pre-existing microorganism contamination on the surface of the aircraft body, bioaerosol sampling was carried out just before takeoff using the same method as atmospheric sampling. Identification was carried out by a homology search for 16S or 18S rDNA isolate sequences in DNA databases (GenBank). Isolate sampling just before takeoff revealed Stretpomyces sp., Micrococcus sp., and Cladosporium sp. One additional strain, Bacillus sp., was isolated from the sample after bioaerosol collection at high altitude. As the microorganism contamination on the aircraft body before takeoff differed from that while in the air, the presence of additional, higher atmosphere-based microorganisms was confirmed. It was found that Bacillus sp. was floating at an altitude of 3,500 m over Noto Peninsula.

引用

页码：164 / 171

页数：7