Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers

被引：0

作者：

S. Goto

R. C. Thakur

K. Ishii

机构：

[1] Fukuoka Prefecture Forest Research and Extension Center,

[2] Toyoda 1438-2,undefined

[3] Yamamoto-machi,undefined

[4] Kurume,undefined

[5] Fukuoka 839-0827,undefined

[6] Japan Fax: (0942) 45-7901 e-mail: Goto<gotos@try-net.or.jp>,undefined

[7] Bio-Resources Technology Division,undefined

[8] Forestry and Forest Products Research Institute,undefined

[9] P.O. Box 16,undefined

[10] Tsukuba Norinkenkyu Danchi-Nai,undefined

[11] Ibaraki 305-8687,undefined

[12] Japan,undefined

来源：

Plant Cell Reports | 1998年 / 18卷

关键词：

Key words Genetic stability; Micropropagation; Pinewood-nematode; Pinus thunbergii; RAPD;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Random amplified polymorphic DNA (RAPD) markers were used to determine the genetic stability of long-term (more than 10 years) micropropagated shoots of Japanese black pine (Pinus thunbergii Parl.). Thirty-six shoots consisting of three morphotypes (short, medium, and long needles) were randomly chosen from about 4,000 micropropagated shoots regenerated from the explants of a single nematode-resistant mother plant. Out of 126 primers screened, 30 gave 134 clear reproducible bands. A total of 4,824 bands obtained from these studies exhibited no aberration in RAPD banding patterns among the tested shoots. Our results show that regenerants from our plant micropropagation system are genetically stable.

引用

页码：193 / 197

页数：4

共 33 条

[1] Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers
Goto, S
Thakur, RC
Ishii, K
PLANT CELL REPORTS, 1998, 18 (3-4) : 193 - 197
[2] Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
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[3] Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers
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[4] Determination of genetic stability of long-term micropropagated plantlets of Platanus acerifolia using ISSR markers
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[5] Identification of the male parents of half-sib progeny from Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard using RAPD markers
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[6] Determination of genetic stability of micropropagated plants of ginger using random amplified polymorphic DNA (RAPD) markers
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[8] An improved micropropagation and assessment of genetic stability of micropropagated Salvadora oleoides using RAPD and ISSR markers
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Acta Physiologiae Plantarum, 2014, 36 : 1115 - 1122
[9] Assessment of genetic stability of micropropagated plants of Rhynchostylis retusa (L.) using RAPD markers
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SCIENTIA HORTICULTURAE, 2021, 281
[10] An improved micropropagation and assessment of genetic stability of micropropagated Salvadora oleoides using RAPD and ISSR markers
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Patel, Ashok Kumar
Rathore, Jitendra Singh
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ACTA PHYSIOLOGIAE PLANTARUM, 2014, 36 (05) : 1115 - 1122

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