Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set

被引：0
|
作者：
Tomáš Csank
Katarína Bhide
Elena Bencúrová
Saskia Dolinská
Petra Drzewnioková
Peter Major
Ľuboš Korytár
Eva Bocková
Mangesh Bhide
Juraj Pistl
机构：
[1] University of Veterinary Medicine and Pharmacy in Košice,Department of Microbiology and Immunology
[2] University of Veterinary Medicine and Pharmacy in Košice,Clinic for Birds and Exotic Animals
[3] University of Veterinary Medicine and Pharmacy in Košice,Department of the Environment, Veterinary Legislation and Economy
[4] University of Veterinary Medicine and Pharmacy in Košice,Department of Epizootology and Parasitology
来源：
Archives of Virology | 2016年 / 161卷
关键词：
West Nile virus; Tick-borne encephalitis virus; Flavivirus; Birds; Raptors;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by sequencing. WNV was detected in swab and brain samples, whereas one brain sample was positive for tick-borne encephalitis virus (TBEV). The WNV sequences clustered with lineages 1 and 2. These results confirm the circulation of WNV in birds in Slovakia and emphasize the risk of infection of humans and horses.
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页码：1679 / 1683
页数：4
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