Effect of Urea on the Enzymatic Hydrolysis of Lignocellulosic Substrate and Its Mechanism

被引：0
|
作者：
Hongming Lou
Meilu Lin
Meijun Zeng
Cheng Cai
Yuxia Pang
Dongjie Yang
Xueqing Qiu
机构：
[1] South China University of Technology,School of Chemistry and Chemical Engineering
[2] South China University of Technology,State Key Laboratory of Pulp and Paper Engineering
来源：
BioEnergy Research | 2018年 / 11卷
关键词：
Lignocellulose; Urea; Enzymatic hydrolysis; Cellulase adsorption;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Effect of hydrogen bond breaker (urea) addition on the enzymatic hydrolysis of Avicel and eucalyptus pretreated by dilute acid (Eu-DA) was investigated. Urea enhanced the enzymatic hydrolysis of Eu-DA at 50 or 30 °C when the concentration of urea was below 60 g/L, while it inhibited the hydrolysis of Avicel. Low concentration urea (< 240 g/L) had little effect on the cellulase spatial structure and its activity. But it decreased cellulase binding to cellulose surface to inhibit the cellulose hydrolysis. Meanwhile, urea obviously prevented the adsorption of cellobiohydrolase I (CBHI) on the lignin in spite of little effect on the adsorption of β-glucosidase (BGL) and two endoglucanases (EGIII and EGV) on lignin. It was proposed that urea enhanced the enzymatic efficiency of Eu-DA by decreasing the cellulase adsorption on lignin surface.
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页码：456 / 465
页数：9
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