Progress and Prospects: The design and production of plasmid vectors

被引：0
|
作者：
D R Gill
I A Pringle
S C Hyde
机构：
[1] University of Oxford,Nuffield Department of Clinical Laboratory Sciences
[2] John Radcliffe Hospital,undefined
[3] The UK Cystic Fibrosis Gene Therapy Consortium,undefined
来源：
Gene Therapy | 2009年 / 16卷
关键词：
non-viral; plasmid; minicircle; promoter; transgene expression;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Plasmid DNA (pDNA) expression vectors are fundamental to all forms of non-viral gene transfer. In this review, we discuss principles of pDNA design and production including the impact of bacterially derived sequences on transgene expression and minicircle approaches to minimize their effects. The impact of inclusion of DNA elements such as scaffold matrix attachment regions (S/MARs), transcription factor (TF)-binding sites and tissue-specific promoters are described. The benefits of eliminating CG dinucleotides (CpGs) from the pDNA are also considered.
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页码：165 / 171
页数：6
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