Differentiation ofFusarium spp. by Fourier Transform Infrared (FT-IR) spectroscopy

被引:0
|
作者
Ming Nie
Kan Bao
Ming Xiao
Jia-Ming Chen
Jiang-Lan Luo
Wei-Qiong Zhang
Jia-Kuan Chen
Bo Li
机构
[1] Shanghai Normal University,College of Life and Environment Sciences
[2] Fudan University,Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, The Institute of Biodiversity Science
来源
Annals of Microbiology | 2006年 / 56卷
关键词
FT-IR spectroscopy; spp.; spore; differentiation; chemometrics;
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中图分类号
学科分类号
摘要
Fourier Transform Infrared (FT-IR) spectroscopy, in combination with chemometrics, was investigated as a simple method to differentiateFusarium spp. Strains were grown on solid culture plates for sporulation. Spectral data of spores ofFusarium spp. were recorded within the range from 4000 to 400 cm−1. Hierarchical cluster analysis (HCA) and principal components analysis (PCA) were used to study the clusters in the data of spectral regions of 3000–2800 and 1800–1480 cm−1. The virtual rate of correct differentiation (100%) was achieved for both the HCA and PCA. FT-IR technique shows high potential as a novel, accurate and easy-to-use differentiation method eagerly sought for the routine differentiation ofFusarium isolates from a wide range of environmental sources.
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页码:399 / 401
页数:2
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