Simultaneous cell cycle and phenotypic analysis of primary uveal melanoma by flow cytometry

被引：0
|
作者：
J Lawry
M O Smith
A J Parsons
I G Rennie
机构：
[1] Institute for Cancer Studies,Department of Ophthalmology
[2] University Medical School,undefined
[3] Royal Hallamshire Hospital,undefined
来源：
Eye | 1998年 / 12卷
关键词：
DNA ploidy; Flow cytometry; Melanoma; Monoclonal antibody;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Background and purpose DNA ploidy and cell cycle measurements of uveal melanoma tissue are regarded as having limited prognostic significance. In contrast, dual-parameter (DNA monoclonal antibody) flow cytometry offers a convenient and rapid way to screen tumour samples for a variety of phenotypic markers, whilst simultaneously measuring DNA ploidy and cell cycle, and therefore has the increased potential to identify clinically relevant indicators of disease progression. The aim of the present study was to identify a simple yet robust method for isolating, preserving and staining cells that could be analysed by flow cytometry.
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页码：431 / 439
页数：8
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