The eureka enzyme: the discovery of DNA polymerase

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Errol C. Friedberg
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[1] Laboratory of Molecular Pathology,the Department of Pathology
[2] University of Texas Southwestern Medical Center,undefined
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The identification and partial purification by Arthur Kornberg and his colleagues in 1956 of an enzyme ? DNA polymerase I of Escherichia coli ? that catalysed the stable incorporation of deoxyribonucleotides into DNA in vitro came as a surprise. At the time, most scientists in the field believed that DNA synthesis was too complicated to be accurately reflected outside the living cell.
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页码:143 / 147
页数:4
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