Direct Determination of β-Hydroxy-β-Methylbutyrate (HMB) in Liquid Nutritional Products

被引：0
|
作者：
Jeffrey H. Baxter
Rosalyn R. Phillips
Lobat Dowlati
Karen C. Goehring
Paul W. Johns
机构：
[1] Abbott Laboratories,
[2] Abbott Nutrition Division,undefined
来源：
Food Analytical Methods | 2011年 / 4卷
关键词：
β-Hydroxy-β-methylbutyrate; HPLC determination; Method validation; Liquid nutritional product; Hydroxy acid; UV absorptivity;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A high performance liquid chromatography/ultraviolet method developed for the simple and direct determination of β-hydroxy-β-methylbutyrate (HMB) in liquid nutritional products and dietary supplements is described. Method suitability was defined by experimental assessments of linearity (r2 > 0.9999), precision (day-to-day RSD <1.5%), accuracy (spike recoveries = 98.1–102.4%, n = 28), selectivity (peak purity average = 99.8 ± 0.8%, n = 10; absence of interference verified by placebo analysis), and quantitation limit (90 mg/kg or 0.8 mM). The method provides for a simple, accurate, and precise quantification of HMB, when present at millimolar concentrations in liquid nutritional products and dietary supplements.
引用
收藏
页码：341 / 346
页数：5
相关论文
共 50 条
  • [1] Direct Determination of β-Hydroxy-β-Methylbutyrate (HMB) in Liquid Nutritional Products
    Baxter, Jeffrey H.
    Phillips, Rosalyn R.
    Dowlati, Lobat
    Goehring, Karen C.
    Johns, Paul W.
    [J]. FOOD ANALYTICAL METHODS, 2011, 4 (03) : 341 - 346
  • [2] Transport Mechanisms for the Nutritional Supplement β-Hydroxy-β-Methylbutyrate (HMB) in Mammalian Cells
    Jiro Ogura
    Toshihiro Sato
    Kei Higuchi
    Yangzom D. Bhutia
    Ellappan Babu
    Masayuki Masuda
    Seiji Miyauchi
    Ricardo Rueda
    Suzette L. Pereira
    Vadivel Ganapathy
    [J]. Pharmaceutical Research, 2019, 36
  • [3] Nutritional supplementation of the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) during resistance training
    Panton, LB
    Rathmacher, JA
    Baier, S
    Nissen, S
    [J]. NUTRITION, 2000, 16 (09) : 734 - 739
  • [4] β-hydroxy-β-methylbutyrate (HMB) production in human subjects
    Walker, Dillon
    Thaden, John
    Engelen, Marielle
    Deutz, Nicolaas
    [J]. FASEB JOURNAL, 2016, 30
  • [5] β-Hydroxy-β-methylbutyrate (HMB) and prevention of muscle wasting
    Hasselgren, Per-Olof
    [J]. METABOLISM-CLINICAL AND EXPERIMENTAL, 2014, 63 (01): : 5 - 8
  • [6] β-hydroxy-β-methylbutyrate (HMB) kinetics and the influence of glucose ingestion in humans
    Vukovich, MD
    Slater, G
    Macchi, MB
    Turner, MJ
    Fallon, K
    Boston, T
    Rathmacher, J
    [J]. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2001, 12 (11): : 631 - 639
  • [7] β-Hydroxy-β-Methylbutyrate (HMB) Supplementation and the Promotion of Muscle Growth and Strength
    Gary J. Slater
    David Jenkins
    [J]. Sports Medicine, 2000, 30 : 105 - 116
  • [8] β-hydroxy-β-methylbutyrate (HMB) supplementation and the promotion of muscle growth and strength
    Slater, GJ
    Jenkins, D
    [J]. SPORTS MEDICINE, 2000, 30 (02) : 105 - 116
  • [9] β-Hydroxy-β-methylbutyrate (HMB) prevents dexamethasone-induced myotube atrophy
    Aversa, Zaira
    Alamdari, Nima
    Castillero, Estibaliz
    Muscaritoli, Maurizio
    Rossi Fanelli, Filippo
    Hasselgren, Per-Olof
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012, 423 (04) : 739 - 743
  • [10] Synergistic effects of polyphenols with leucine and β-hydroxy- β-methylbutyrate (HMB) on energy metabolism
    Bruckbauer, Antje
    Baggett, Brooke
    Zemel, Michael B.
    [J]. FASEB JOURNAL, 2013, 27
12345