Investigation of mealybug (hemiptera: Coccoidea: Pseudococcidae) species from Turkey by RAPD

被引：0
|
作者：
Ç. Ulubaş Serçe
M. B. Kaydan
A. N. Kilinçer
F. Ertunç
机构：
[1] Mustafa Kemal University,Plant Protection Department, Agriculture Faculty
[2] Yüzüncü Yıl University,Plant Protection Department, Agriculture Faculty
[3] Ankara University,Plant Protection Department, Agriculture Faculty
来源：
Phytoparasitica | 2007年 / 35卷
关键词：
Mealyburg; RAPD;
D O I：
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摘要：
Molecular markers are commonly used for determining the relationship between insect species. In this study, we investigated the relationship of six mealybug species,Planococcus citri (Risso),Planococcus ficus (Signoret),Planococcus vovae (Nasonov),Pseudococcus longispinus (Targioni Tozzetti),Pseudococcus viburni (Signoret) andPhenacoccus aceris (Signoret), sampled from Turkey, using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). Of the 50 RAPD primers tested, 18 identified a total of 256 fragments of which all were polymorphic. Sufficient discrimination betweenP. citri andP. ficus was provided by several RAPD primers. The genetic distance values calculated for each pair-wise comparison ranged from 0.156 to 0.504. Cluster analyses of RAPD data clearly separated the species into two groups.
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