Biologically Active Human Interferon α-2b Produced in the Egg White of Transgenic Hens

被引：0
|
作者：
Jeffrey C. Rapp
Alex J. Harvey
Gordon L. Speksnijder
Wei Hu
Robert Ivarie
机构：
[1] AviGenics,Department of Genetics
[2] Inc.,undefined
[3] Georgia BioBusiness Center,undefined
[4] University of Georgia,undefined
来源：
Transgenic Research | 2003年 / 12卷
关键词：
bioreactor; chickens; glycosylation; human interferon; recombinant protein; transgenesis;
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中图分类号：
学科分类号：
摘要：
We have previously described the expression of a bacterial protein in the egg white of transgenic chickens using a replication-deficient retroviral vector. Here we report the expression of a glycosylated human protein, interferon α-2b (hIFN), in the egg white of transgenic hens. The hIFN secreted into the egg white was biologically active as determined by a viral inhibition assay. Purification and carbohydrate analysis of the hIFN expressed in egg white revealed that two of the six major glycosylated hIFN species match the naturally occurring human hIFN glycovariants. These results support the potential of the hen as a bioreactor for the production of commercially valuable, biologically active, and glycosylated proteins in egg white.
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页码：569 / 575
页数：6
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