Species of Asterina are plant parasitic, bitunicate Ascomycetes which are particularly diverse in tropical regions. In the present publication, detailed descriptions, keys and illustrations of two newly described species and five newly recorded species of Asterina recently collected in Panama are presented. New species are Asterina gaiadendricola on Gaiadendron punctatum (Loranthaceae) and Asterina schlegeliae on Schlegelia parviflora (Schlegeliaceae) with their respective anamorphic Asterostomella and Mahanteshamyces states. New records for Panama are A. manihotis, A. ciferriana from a new host plant species (Caesalpinia bonduc, Fabaceae s.l.), A. consobrina from a new host plant species (Solanum aphyodendron, Solanaceae), A. fuchsiae from a new host plant species (Fuchsia paniculata, Onagraceae) and A. phenacis from a new host plant species (Phenax mexicanus, Urticaceae). A. ciferriana, A. consobrina, A. fuchsiae, A. manihotis and A. phenacis are illustrated for the first time.