Ozone oxidation pretreatment for enzymatic saccharification of spent culture media after Lentinula edodes cultivation

Ozone oxidation pretreatment was carried out on spent culture media (SCMs) after Lentinula edodes cultivation to improve fermentable sugar production by enzymatic saccharification. SCM samples treated with ozone for different treatment time under various moisture contents (MCs) were enzymatically saccharified, and hydrolysis weight decrease, reducing sugar yield, and monosaccharide yields were determined. Klason lignin content in SCM samples with 20 and 40 % MC was greatly decreased by ozone pretreatment for all ozone treatment time. In addition, in the ozone-treated sample at 40 % MC, the highest hydrolysis weight decrease after saccharification was obtained in the SCM sample treated for 1 h. After saccharification, glucose yield from the ozone-treated sample for 1 h was higher than that treated for 2 h. These results indicate that ozone treatment is effective to improve fermentable sugar production by enzymatic saccharification for the SCMs of L. edodes.