Effects of repeated thermal manipulation of broiler embryos on hatchability, chick quality, and post-hatch performance

The current study aimed to evaluate the effects of embryonic thermal manipulation (TM) on hatching criteria, chick quality, and subsequent growth performance of broiler chickens under heat stress (HS) condition. Two thousand fertile eggs were randomly divided between 2 groups and incubated under standard (37.8 °C and 56% relative humidity (RH)) and TM (39.5 °C and 65% RH) conditions. Temperature and humidity were identical in both groups within the first 10 days. The eggs in the TM group were exposed to 39.5 °C and 65% RH for 3 h/day from 11 to 16 days of incubation. Egg weight (EW) was measured in 1, 11, and 18 days of incubation, and eggshell temperature (EST) was recorded daily. Chick quality was, also, evaluated according to the Tona method on hatch day. Samples of the chicks (n = 20) were euthanized and dissected at 0-day post-hatch, and different carcass parts were weighed, and blood samples were collected for hormones analysis. The post-hatch growth performance of both groups was also recorded under HS (37 °C for 5 h beginning at 22 days) condition. The results showed that TM did not significantly affect hatchability and embryonic mortality (P > 0.05). The female chick percentage was higher in the TM group (P < 0.05). Eggshell temperature and serum concentrations of corticosterone and T4 were significantly higher in the TM compared with the control chicks (P < 0.05). The chick length was considerably shorter in TM chicks (P < 0.05). Chick quality was not influenced by TM. There was no significant difference between the two groups in the post-hatch growth performance (P > 0.05). In conclusion, exposing broiler embryos to the controlled TM did not have adverse effects on chick quality and post-hatch growth performance.