Effects of stromal-derived factor 1 preconditioning on apoptosis of rat bone mesenchymal stem cells

被引：0

作者：

Jun Chen

Xinling Du

Kailun Zhang

机构：

[1] Huazhong University of Science and Technology,Department of Cardiac Surgery, Union Hospital, Tongji Medical College

来源：

Journal of Huazhong University of Science and Technology [Medical Sciences] | 2009年 / 29卷

关键词：

bone mesenchymal stem cells; SDF-1; preconditioning; apoptosis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The effects of stromal-derived factor 1 preconditioning (PC) on apoptosis of bone mesenchymal stem cells (BMSCs) treated with hypoxia plus serum deprivation were investigated. Bone mesenchymal stem cells were cultured with the whole marrow-adherence technique. RT-PCR and immunohistochemistry were used to detect the expression of CXCR4. BMSCs were incubated in medium for 24 h with 10 ng/mL and 100 ng/mL SDF-1 respectively, and then they were treated with hypoxia plus serum deprivation for 6 h. Apoptosis rate was determined by flow cytometry and TUNEL method. The results showed that BMSCs had CXCR4 expression. The number of apoptotic cells was significantly reduced in SDF-1 PC group as compared with the control group, and 100 ng/mL SDF-1 PC group had the lowest level of apoptosis. It was concluded that SDF-1 preconditioning suppresses the apoptosis of BMSCs treated with hypoxia plus serum deprivation.

引用

页码：423 / 426

页数：3

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