Joint profiling of DNA methylation and chromatin architecture in single cells

被引：0
|
作者：
Guoqiang Li
Yaping Liu
Yanxiao Zhang
Naoki Kubo
Miao Yu
Rongxin Fang
Manolis Kellis
Bing Ren
机构：
[1] Ludwig Institute for Cancer Research,Division of Human Genetics
[2] Cincinnati Children’s Hospital Medical Center,Department of Pediatrics
[3] University of Cincinnati College of Medicine,Bioinformatics and Systems Biology Graduate Program
[4] Cincinnati Cancer Center,Department of Cellular and Molecular Medicine, Center for Epigenomics
[5] University of Cincinnati,undefined
[6] University of California,undefined
[7] San Diego,undefined
[8] Broad Institute of MIT and Harvard,undefined
[9] Massachusetts Institute of Technology,undefined
[10] Computer Science and Artificial Intelligence Laboratory,undefined
[11] Institute of Genomic Medicine,undefined
[12] Moores Cancer Center,undefined
[13] University of California,undefined
[14] San Diego,undefined
[15] School of Medicine,undefined
来源：
Nature Methods | 2019年 / 16卷
关键词：
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学科分类号：
摘要：
We report a molecular assay, Methyl-HiC, that can simultaneously capture the chromosome conformation and DNA methylome in a cell. Methyl-HiC reveals coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus, and delineates heterogeneity of both the chromatin architecture and DNA methylome in a mixed population. It enables simultaneous characterization of cell-type-specific chromatin organization and epigenome in complex tissues.
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页码：991 / 993
页数：2
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