The centrosomal localization of KM-HN-1 (MGC33607) depends on the leucine zipper motif and the C-terminal coiled-coil domain

被引：0
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作者：
Hye Jeong Park
Hyun-Joo Seo
Hyun-Woo Kim
Jung Soon Kim
So-Yoon Hwang
Yeon-Sun Seong
机构：
[1] Dankook University,Department of Biochemistry, College of Medicine
来源：
Experimental & Molecular Medicine | 2007年 / 39卷
关键词：
CDC110 protein, human; cell nucleus; centrosome; protein structure, tertiary; protein transport;
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学科分类号：
摘要：
KM-HN-1 is a C-terminal coiled-coil domain containing protein previously referred to as image clone MGC33607. This protein has been previously identified as a cancer/testis antigen and reported as nuclear and chromatin localizing protein. We raised polyclonal antisera with the GST fusion protein and identified them as a 105 kDa protein. Motif analysis showed that this protein harbors the leucine zipper motif in internal 1/3 region and the coiled-coil domain in the C-terminal region. Using the full length and various deletion mutants, we determined the motif that governs the subcellular localization of KM-HN-1. Immunofluorescence staining of the endogenous KM-HN-1 and various kinds of GFP-tagged KM-HN-1 revealed that KM-HN-1 localizes to the centrosomes as well as nucleus. The centrosomal localization-determining region of this protein is C-terminal coiled-coil domain in which the leucine zipper motif and the nuclear export signal (NES) harbor.
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页码：828 / 838
页数：10
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