Proteome-wide identification of SUMO modification sites by mass spectrometry

被引：0
|
作者：
Triin Tammsalu
Ivan Matic
Ellis G Jaffray
Adel F M Ibrahim
Michael H Tatham
Ronald T Hay
机构：
[1] Centre for Gene Regulation and Expression,
[2] Sir James Black Centre,undefined
[3] College of Life Sciences,undefined
[4] University of Dundee,undefined
[5] MRC Protein Phosphorylation and Ubiquitination Unit,undefined
[6] Sir James Black Centre,undefined
[7] College of Life Sciences,undefined
[8] University of Dundee,undefined
[9] Present address: Max Planck Institute for Biology of Ageing,undefined
[10] Cologne,undefined
[11] Germany.,undefined
来源：
Nature Protocols | 2015年 / 10卷
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学科分类号：
摘要：
Sumoylation is a post-translational modification involved in regulating many cellular processes. This protocol is for detecting sumoylation sites in cultured cells using a diGly-Lys (K-ɛ-GG)–specific antibody and mass spectrometry–based proteomics.
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收藏
页码：1374 / 1388
页数：14
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