Effects of Corn Straw and Citric Acid on Removal of PAHs in Contaminated Soil Related to Changing of Bacterial Community and Functional Gene Expression

Root exudates can stimulate microbial degradation in rhizosphere, but it is unclear whether the rhizodegradation of polycyclic aromatic hydrocarbons (PAHs) occurs in corn straw-amended soil. Either citric acid or corn straw was added into PAHs-contaminated soil to investigate their effect on the removal of PAHs. Either corn straw (Y) or combined application of corn straw and citric acid (YN100) significantly (p < 0.05) enhanced the removal of soil PAHs by 8.43% and 18.62%, respectively. Both Y and YN100 treatments obviously increased the abundance of PAHs degraders and the potential hosts of PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes. Interestingly, the copies of PAH-RHDα Gram-negative bacteria genes under YN100 treatment was significantly (p < 0.05) higher than those under Y treatment. The present results indicated that combined application of corn straw and citric acid could efficiently enhance the removal of PAHs in soil, mainly via increasing the relative abundances of PAH-degrading bacteria and the expression of PAH-RHDα genes in contaminated soil.