Solid-phase synthesis of pseudo-complementary peptide nucleic acids

被引：0
|
作者：
Makoto Komiyama
Yuichiro Aiba
Takumi Ishizuka
Jun Sumaoka
机构：
[1] Research Center for Advanced Science and Technology,
[2] The University of Tokyo,undefined
[3] 4-6-1 Komaba,undefined
[4] Meguro-ku,undefined
来源：
Nature Protocols | 2008年 / 3卷
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摘要：
Pseudo-complementary peptide nucleic acid (pcPNA) is a DNA analog in which modified DNA bases 2,6-diaminopurine (D) and 2-thiouracil (Us) 'decorate' a poly[N-(2-aminoethyl)glycine] backbone, together with guanine (G) and cytosine (C). One of the most significant characteristics of pcPNA is its ability to effect double-duplex invasion of predetermined DNA sites inducing various changes in the biological and the physicochemical properties of the DNA. This protocol describes solid-phase synthesis of pcPNA. The monomers for G and C are commercially available, but the monomers for D and Us need to be synthesized (or can be ordered to custom synthesis companies). Otherwise, the procedure is the same as that employed for Boc-strategy synthesis of conventional PNA. This protocol, if the synthesis of D and Us monomers is not factored in, takes approximately 7 d to complete.
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页码：646 / 654
页数：8
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