Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

被引：0
|
作者：
Marian AC Piqueur
Walter A Verstrepen
Peggy Bruynseels
An H Mertens
机构：
[1] ZNA Hospitals,Department of Microbiology
[2] site Middelheim,undefined
来源：
Virology Journal | / 6卷
关键词：
Aseptic Meningitis; Proficiency Panel; Exonuclease Digestion; Enterovirus Serotypes; Enterovirus Genome;
D O I：
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摘要：
We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.
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