Mapping biological composition through quantitative phase and absorption X-ray ptychography

被引：0

作者：

Michael W. M. Jones

Kirstin Elgass

Mark D. Junker

Mac B. Luu

Michael T. Ryan

Andrew G. Peele

Grant A. van Riessen

机构：

[1] ARC Centre of Excellence for Coherent X-Ray Science,Department of Physics

[2] La Trobe University,Department of Biochemistry

[3] ARC Centre of Excellence for Coherent X-Ray Science,Department of Biochemistry and Molecular Biology

[4] La Trobe University,ARC Centre of Excellence for Advanced Molecular Imaging

[5] Australian Synchrotron,undefined

[6] ARC Centre of Excellence for Advanced Molecular Imaging,undefined

[7] Australian Synchrotron,undefined

[8] Australian Synchrotron,undefined

[9] Monash Institute of Medical Research,undefined

[10] Monash Micro Imaging,undefined

[11] Chu Van An Gifted High School,undefined

[12] Monash University,undefined

[13] Australian Synchrotron,undefined

来源：

Scientific Reports | / 4卷

关键词：

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摘要：

Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.

引用

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[J]. SCIENTIFIC REPORTS, 2014, 4
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