CRISPR-UMI: single-cell lineage tracing of pooled CRISPR–Cas9 screens

被引：0

作者：

Georg Michlits

Maria Hubmann

Szu-Hsien Wu

Gintautas Vainorius

Elena Budusan

Sergei Zhuk

Thomas R Burkard

Maria Novatchkova

Martin Aichinger

Yiqing Lu

John Reece-Hoyes

Roberto Nitsch

Daniel Schramek

Dominic Hoepfner

Ulrich Elling

机构：

[1] Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA),Department of Molecular Genetics

[2] Vienna Biocenter (VBC),undefined

[3] Research Institute of Molecular Pathology (IMP),undefined

[4] Vienna Biocenter (VBC),undefined

[5] ,undefined

[6] Center for Systems Biology,undefined

[7] Lunenfeld-Tanenbaum Research Institute,undefined

[8] Mount Sinai Hospital,undefined

[9] University of Toronto,undefined

[10] Novartis Institutes for BioMedical Research,undefined

[11] Discovery Sciences RAD,undefined

[12] AstraZeneca R&D,undefined

[13] Novartis Institutes for BioMedical Research,undefined

来源：

Nature Methods | 2017年 / 14卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Combining each sgRNA with a unique molecular identifier in a genome-wide screen increases sensitivity and robustness in both positive and negative selection.

引用

页码：1191 / 1197

页数：6

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