Transcriptome characterization and sequencing-based identification of drought-responsive genes in potato

被引:0
|
作者
Ning Zhang
Bailin Liu
Congyu Ma
Guodong Zhang
Jing Chang
Huaijun Si
Di Wang
机构
[1] Gansu Agricultural University,Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement
[2] Gansu Agricultural University,College of Life Science and Technology
来源
Molecular Biology Reports | 2014年 / 41卷
关键词
Potato; mRNA-Seq; Transcriptome; Drought stress; Leaf;
D O I
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中图分类号
学科分类号
摘要
Potato (Solanum tubersosum L.) is relatively vulnerable to abiotic stress conditions such as drought, but the tolerance mechanisms to such stress in potato are largely unknown. To gain insight into the transcriptome dynamics that are associated with drought stress, genome-wide gene expression profile was conducted by Solexa sequencing to generate a large dataset and a comprehensive transcriptome profile for potato. Here, we report a reference for the potato transcriptome using leaf tissues under drought-stressed condition from a local potato cultivar ‘Longshu 3’. Analysis of 86,965,482 RNA-Seq reads permitted the detection and quantification of expression levels of 7,284 genes at transcriptional levels, among them, 6,754 genes were enriched in draught-treated leaves while 6,419 in control. We identified 842 drought-responsive up-regulated and 494 down-regulated candidate genes with significantly differentially expression under continued drought stress treatments. Those differently expressed genes were mostly enriched in 89 gene categories and 21 KEGG pathways. Drought-stressed leaves had increased expression of genes involved in stress response compared with control leaves. A subset of differentially expressed genes associated with drought response was examined using quantitative real-time PCR. These results provide a broad spectrum of candidate genes that are essential for understanding the molecular regulation of potato in response to abiotic stresses.
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页码:505 / 517
页数:12
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