Identification of a Strong Promoter of Bacteriophage MB78 that Interacts with a Host Coded Factor and Regulates the Expression of a Structural Protein

被引：0
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作者：
Manzoor Ahmad Zargar
Deepti Chaturvedi
Maharani Chakravorty
机构：
[1] *Molecular Biology Unit,
[2] Institute of Medical Sciences,undefined
[3] Banaras Hindu University,undefined
[4] Varanasi—221005,undefined
[5] U.P. †India and National Institute of Cholera and Enteric Diseases,undefined
来源：
Virus Genes | 2001年 / 22卷
关键词：
strong promoter; bacteriophage MB78; DNA binding proteins; regulatory protein; host coded factor; bacteriophage structural protein;
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摘要：
A strong promoter of bacteriophage MB78 which controls the expression of a small structural protein of the phage has been identified and characterized. Analysis of its nucloetide sequence upstream of the translational start site revealed the presence of conserved −10 (TAATAT) and −35 (TTCTCCT) regions. It was observed that transcription initiates with thymidine residue, 86 nt upstream of the translational start site. Transcription seems to undergo alternate up and down regulation. This promoter is efficiently recognized by sigma 70 RNA polymerase and a host factor also binds to it. Binding of RNA polymerase is independent of binding of the host factor.
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页码：35 / 45
页数：10
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