A new cell line from larval fat bodies of the bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)

被引：0
|
作者：
Huan Zhang
Yong-An Zhang
Qilian Qin
Yuzhu Wang
Xuan Li
Lin Miao
Zhenxian Yin
Aijun Zhang
Liangjian Qu
Cui Ding
机构：
[1] Chinese Academy of Forestry,Key Lab of Forest Protection of State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection
[2] Chinese Academy of Sciences,State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology
来源：
In Vitro Cellular & Developmental Biology - Animal | 2006年 / 42卷
关键词：
fat body; cell line; DAF-PCR; karyotype;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A new cell line, designated IOZCAS-Ha-I, was initiated from the fat body of larvae of Helicoverpa armigera (Lepidoptera: Noctuidae) in TNM-FH medium containing 10% fetal bovine serum. Spherical cells were predominant among the various cell types. The cell line showed a typical lepidopteran chromosome pattern ranging from 58 to 239 chromosomes in the majority of the cells, it was confirmed to have originated from the H. armigera by the DNA amplification-fingerprinting polymerase chain reaction (DAF-PCR) technique. The new cell line was only slightly susceptible to the multiple nucleocapsid nuclear polyhedrosis viruses (NPV) from H. armigera.
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页码：290 / 293
页数：3
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