Imobilization of β-glucosidase using the cellulose-binding domain of Bacillus subtilis endo-β-1,4-glucanase

被引：0
|
作者：
Dong Ho Ahn
Hoon Kim
Moo Young Pack
机构：
[1] R Center,Department of Agricultural Chemistry
[2] Cheiljedang Cooperation,undefined
[3] Sunchon National University,undefined
[4] Department of Biotechnology,undefined
[5] KAIST,undefined
来源：
Biotechnology Letters | 1997年 / 19卷
关键词：
Fusion Protein; Cellulase; Cellobiose; Avicel; Cellulomonas;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A recombinant plasmid pβCBD was constructed for immobilization of Cellulomonas fimi β-glucosidase (Cbg) using the cellulose-binding domain (CBD) of Bacillus subtilis BSE 616 endo-β-1,4-glucanase (Beg). The Cbg-CBD Beg fusion protein, 80 kDa, was expressed in Escherichia coli and immobilized to Avicel. Cellobiose was completely hydrolyzed with the immobilized fusion protein. The fusion protein bound to Avicel retained full activity during continuous operation for 24 h at 4°C.
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页码：483 / 486
页数：3
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