A rapid one-step multiplex RT-PCR assay for the simultaneous detection of five citrus viroids in China

被引：1
|
作者：
Xuefeng Wang
Changyong Zhou
Kezhi Tang
Yan Zhou
Zhongan Li
机构：
[1] Southwest University,National Citrus Virus Exclusion Center, Citrus Research Institute
来源：
European Journal of Plant Pathology | 2009年 / 124卷
关键词：
Citrus viroids; Multiplex; One-step RT-PCR; Nucleic acid extraction method;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Citrus plants are natural hosts of five viroid species and large numbers of sequence variants. In this paper a simple and sensitive one step multiplex RT-PCR protocol with an internal control was utilised to simultaneously detect and differentiate five citrus viroids: Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd), Hop stunt viroid (HSVd), Citrus viroid-III (CVd-III) and Citrus viroid-IV (CVd-IV). In addition, a micro and rapid total nucleic acid extraction method was developed and the protocol applied to evaluate the occurrence and distribution of citrus viroids in China.
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收藏
页码：175 / 180
页数：5
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