Guanosine enhances glutamate uptake in brain cortical slices at normal and excitotoxic conditions

被引：0
|
作者：
Dos Santos Frizzo M.E. [1 ,2 ]
Lara D.R. [3 ]
Prokopiuk A.D.S. [1 ]
Vargas C.R. [4 ]
Salbego C.G. [1 ]
Wajner M. [1 ]
Souza D.O. [1 ]
机构：
[1] Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre
[2] Departamento de Cincias Morfológicas, Universidade Federal do Rio Grande do Sul, Porto Alegre
[3] Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre
[4] Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre
来源：
Cellular and Molecular Neurobiology | 2002年 / 22卷 / 3期
关键词：
Excitotoxicity; Glutamate; Hypoglycemia; Hypoxia; Neuroprotection; Taurine;
D O I：
10.1023/A:1020728203682
中图分类号：
学科分类号：
摘要：
1. The effect of guanosine on L-[2,3-3H]glutamate uptake was investigated in brain cortical slices under normal or oxygen-glucose deprivation (OGD) conditions. 2. In slices exposed to physiological conditions, guanosine (1-100 μM) stimulated glutamate uptake (up to 100%) in a concentration-dependent manner when a high (100 μM) but not a low (1 μM) concentration of glutamate was used. 3. In slices submitted to OGD, guanosine 1 and 100 μM also increased 100 μM glutamate uptake (38 and 70%, respectively). 4. The increasing of glutamate and taurine released to the incubation medium in cortical slices submitted to OGD were significantly attenuated by the presence of guanosine in the incubation medium. 5. Guanosine prevented the increase in propidium iodide incorporation into cortical slices induced by OGD, indicating a protective role against ischemic injury. 6. These results support the hypothesis of a protective role for guanosine during brain ischemia, possibly by activating glutamate uptake into neural cells.
引用
收藏
页码：353 / 363
页数：10
相关论文
共 50 条
  • [1] Guanosine enhances glutamate uptake in brain cortical slices at normal and excitotoxic conditions
    Frizzo, MED
    Lara, DR
    Prokopiuk, AD
    Vargas, CR
    Salbego, CG
    Wajner, M
    Souza, DO
    CELLULAR AND MOLECULAR NEUROBIOLOGY, 2002, 22 (03) : 353 - 363
  • [2] Guanosine enhances glutamate transport capacity in brain cortical slices
    Frizzo, ME
    Schwalm, FD
    Frizzo, JK
    Soares, FA
    Souza, DO
    CELLULAR AND MOLECULAR NEUROBIOLOGY, 2005, 25 (05) : 913 - 921
  • [3] Guanosine Enhances Glutamate Transport Capacity in Brain Cortical Slices
    Marcos Emílio Frizzo
    Fábio Duarte Schwalm
    Juliana Karl Frizzo
    Félix Antunes Soares
    Diogo Onofre Souza
    Cellular and Molecular Neurobiology, 2005, 25 : 913 - 921
  • [4] Ontogenetic profile of glutamate uptake in brain structures slices from rats: sensitivity to guanosine
    Thomazi, AP
    Godinho, GFRS
    Rodrigues, JM
    Schwalm, FD
    Frizzo, MES
    Moriguchi, E
    Souza, DO
    Wofchuk, ST
    MECHANISMS OF AGEING AND DEVELOPMENT, 2004, 125 (07) : 475 - 481
  • [5] Guanosine enhances glutamate uptake and oxidation, preventing oxidative stress in mouse hippocampal slices submitted to high glutamate levels
    Nonose, Y.
    Pieper, L. Z.
    da Silva, J. S.
    Longoni, A.
    Apel, R., V
    Meira-Martins, L. A.
    Grings, M.
    Leipnitz, G.
    Souza, D. O.
    de Assis, A. M.
    BRAIN RESEARCH, 2020, 1748
  • [6] [H-3] GLUTAMATE UPTAKE INTO CEREBRAL CORTICAL SLICES
    YAMADA, K
    OGITA, K
    YONEDA, Y
    JAPANESE JOURNAL OF PHARMACOLOGY, 1987, 43 : P263 - P263
  • [7] Ischemic and excitotoxic damage to brain slices from normal and microencephalic rats
    Virgili, M
    Vandi, M
    Contestabile, A
    NEUROSCIENCE LETTERS, 1997, 233 (01) : 53 - 57
  • [8] Profile of glutamate uptake in hippocampal slices exposed to oxygen and glucose deprivation: guanosine protection
    Thomazi, Ana Paula
    Boff, Bruna
    Pires, Tales
    Godinho, Graca
    Battu, Cintia
    Gottfried, Carmem
    Souza, Diogo
    Salbego, Christiane
    Wofchuk, Susana
    NEURON GLIA BIOLOGY, 2007, 2 : S159 - S159
  • [9] Nicergoline enhances glutamate uptake via glutamate transporters in rat cortical synaptosomes
    Nishida, A
    Iwata, H
    Kudo, Y
    Kobayashi, T
    Matsuoka, Y
    Kanai, Y
    Endou, H
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2004, 27 (06) : 817 - 820
  • [10] Rutin improves glutamate uptake and inhibits glutamate excitotoxicity in rat brain slices
    Ferreira, Rafael S.
    Teles-Souza, Jessica
    dos Santos Souza, Cleide
    Pereira, Erica P. L.
    de Araujo, Fillipe M.
    da Silva, Alessandra Bispo
    Castro e Silva, Juliana H.
    Nonose, Yasmine
    Nunez-Figueredo, Yanier
    de Assis, Adriano M.
    Souza, Diogo O.
    Costa, Maria de Fatima D.
    Moreira, Jose Claudio F.
    Costa, Silvia L.
    da Silva, Victor D. A.
    MOLECULAR BIOLOGY REPORTS, 2021, 48 (02) : 1475 - 1483
12345