Influence of rhizobial (Rhizobium leguminosarum) inoculation and calcium ions on the NADPH oxidase activity in roots of etiolated pea (Pisum sativum L.) seedlings

被引：0
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作者：
A. K. Glyan’ko
A. A. Ischenko
机构：
[1] Russian Academy of Sciences,Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch
来源：
Applied Biochemistry and Microbiology | 2013年 / 49卷
关键词：
Apply Biochemistry; Rhizobium; NADPH Oxidase; Daidzein; NADPH Oxidase Activity;
D O I：
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学科分类号：
摘要：
Changes in the functional activity of the NADPH oxidase in the microsomal fraction of roots of etiolated pea seedlings, caused by rhizobial inoculation and calcium ions (Ca2+), are shown. The enzyme activity in a medium with an exogenous source of Ca2+ (CaCl2, 100 μM) fluctuated, increasing 5 to 20 min and decreasing 10 and 30 min after addition. A calcium chelator (ethylene glycol tetraacetic acid (EDTA), 100 μM) potentiated the decrease in the enzyme activity in the presence of exogenous calcium. Rhizobial inoculation caused a 3.9-fold increase in the enzyme activity 5 min after inoculation compared to the control (without inoculation). The Ca2+-channel activator (amiodarone, 300 μM) and the Ca2+-channel blocker (lanthanum chloride, 400 μM) reduced the NADPH oxidase activity after rhizobial inoculation compared to the control level (without inoculation). It is concluded that Ca2+ and reactive oxygen species are involved in the regulation of the membrane NADPH oxidase activity in roots of pea seedlings.
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页码：215 / 219
页数：4
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