A search for protein-protein interactions of peroxiredoxin 6 with the yeast two-hybrid system

被引：0
|
作者：
E. N. Budanova
M. F. Bystrova
机构：
[1] Russian Academy of Sciences,Institute of Cell Biophysics
来源：
Russian Journal of Genetics | 2008年 / 44卷
关键词：
Chimeric Protein; Hybrid Plasmid; Antioxidant Redox Signaling; LexA Protein; Thiol Peroxidase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Peroxiredoxins (Prx) are a family of nonselenium peroxidases that are involved in cell defense against oxidative stress and in redox regulation of intracellular signaling. Mammalian peroxiredoxin 6 (Prx6) belongs to the 1-Cys Prx subfamily. The protein-protein interactions of human Prx6 were studied using a yeast two-hybrid system. Hybrid plasmid pHybLex/Zeo/Prx6, which directed synthesis of a chimeric protein consisting of the DNA-binding domain (BD) of LexA and a Prx6 sequence, was used to screen a two-hybrid cDNA library Hybrid Hunter (Invitrogen). The screening identified two potential interaction partners of Prx6: the calcium-activated cysteine endopeptidase calpain and the p50RhoGAP protein of the family of Sec14-like proteins. The possibility for the interactions observed in the two-hybrid system to occur in oxidative stress in vivo is discussed.
引用
收藏
相关论文
共 50 条
  • [1] A search for protein-protein interactions of peroxiredoxin 6 with the yeast two-hybrid system
    Budanova, E. N.
    Bystrova, M. F.
    [J]. RUSSIAN JOURNAL OF GENETICS, 2008, 44 (02) : 137 - 142
  • [2] Analysing protein-protein interactions with the yeast two-hybrid system
    Causier, B
    Davies, B
    [J]. PLANT MOLECULAR BIOLOGY, 2002, 50 (06) : 855 - 870
  • [3] Analysing protein-protein interactions with the yeast two-hybrid system
    Barry Causier
    Brendan Davies
    [J]. Plant Molecular Biology, 2002, 50 : 855 - 870
  • [4] The yeast two-hybrid system for identifying protein-protein interactions
    Coates, PJ
    Hall, PA
    [J]. JOURNAL OF PATHOLOGY, 2003, 199 (01): : 4 - 7
  • [5] Two-hybrid analysis of protein-protein interactions in yeast
    Rosamond, J
    [J]. YEAST GENE ANALYSIS, 1998, 26 : 255 - 268
  • [6] Determination of protein-protein interactions of ICln by the yeast two-hybrid system
    Schmarda, A
    Fresser, F
    Gschwentner, M
    Fürst, J
    Ritter, M
    Lang, F
    Baier, G
    Paulmichl, M
    [J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2001, 11 (01) : 55 - 60
  • [7] Protein-protein interactions in two potyviruses using the yeast two-hybrid system
    Lin, Lin
    Shi, Yuhong
    Luo, Zhaopeng
    Lu, Yuwen
    Zheng, Hongying
    Yan, Fei
    Chen, Jiong
    Chen, Jianping
    Adams, M. J.
    Wu, Yunfeng
    [J]. VIRUS RESEARCH, 2009, 142 (1-2) : 36 - 40
  • [8] Protein-protein interactions between keratin polypeptides expressed in the yeast two-hybrid system
    Schnabel, J
    Weber, K
    Hatzfeld, M
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1998, 1403 (02): : 158 - 168
  • [9] A yeast two-hybrid approach for probing protein-protein interactions at the centrosome
    Galletta, Brian J.
    Rusan, Nasser M.
    [J]. CENTROSOME & CENTRIOLE, 2015, 129 : 251 - 277
  • [10] Combined transformation and genetic technique verification of protein-protein interactions in the yeast two-hybrid system
    Tyagi, S
    Lal, SK
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 277 (03) : 589 - 593
12345