Coat protein-mediated resistance to Turnip mosaic virus in oilseed rape (Brassica napus)

被引:0
|
作者
Przemyslaw Lehmann
Carol E. Jenner
Edward Kozubek
Andrew J. Greenland
John A. Walsh
机构
[1] Polish Academy of Sciences,Institute of Plant Genetics
[2] Horticulture Research International,undefined
[3] Syngenta,undefined
来源
Molecular Breeding | 2003年 / 11卷
关键词
Coat protein-mediated resistance; Oilseed rape; Pathogen-derived resistance; TuMV;
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学科分类号
摘要
Oilseed rape (Brassica napus) lines transformedwith the coat protein (CP) gene of Turnip mosaic virus(TuMV) were used to determine the effectiveness of resistance to TuMV mediatedby CP RNA or coat protein. Lines with one, two, or more copies of transgeneswere produced. T2 and T3 lines containing the CP genewitha functional start codon synthesised coat protein and showed high, but variablelevels of resistance to TuMV (21–96% resistant plants per line). TheT1 and T2 progeny of all lines carrying the CP gene withamutated start codon so that RNA but not protein was expressed, were assusceptible to TuMV as controls. Thus, in these experiments we were able toinduce CP-mediated resistance, but not RNA-mediated resistance.
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页码:83 / 94
页数:11
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