Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats

被引：0
|
作者：
Alexandr Klioutchnikov
Damian J. Wallace
Michael H. Frosz
Richard Zeltner
Juergen Sawinski
Verena Pawlak
Kay-Michael Voit
Philip St. J. Russell
Jason N. D. Kerr
机构：
[1] Research Center Caesar,Department of Behavior and Brain Organization
[2] Max Planck Institute for the Science of Light,undefined
来源：
Nature Methods | 2020年 / 17卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 neuronal activity for >1 h in freely moving rats performing a range of behaviors.
引用
收藏
页码：509 / 513
页数：4
相关论文
共 50 条
  • [1] Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats
    Klioutchnikov, Alexandr
    Wallace, Damian J.
    Frosz, Michael H.
    Zeltner, Richard
    Sawinski, Juergen
    Pawlak, Verena
    Voit, Kay-Michael
    Russell, Philip St. J.
    Kerr, Jason N. D.
    [J]. NATURE METHODS, 2020, 17 (05) : 509 - +
  • [2] A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice
    Alexandr Klioutchnikov
    Damian J. Wallace
    Juergen Sawinski
    Kay-Michael Voit
    Yvonne Groemping
    Jason N. D. Kerr
    [J]. Nature Methods, 2023, 20 : 610 - 616
  • [3] A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice
    Klioutchnikov, Alexandr
    Wallace, Damian J.
    Sawinski, Juergen
    Voit, Kay-Michael
    Groemping, Yvonne
    Kerr, Jason N. D.
    [J]. NATURE METHODS, 2023, 20 (04) : 610 - 616
  • [4] Detachable head-mounted photoacoustic microscope in freely moving mice
    Heng, Guo
    Chen, Qian
    Qin, Wei
    Qi, Weizhi
    Xi, Lei
    [J]. OPTICS LETTERS, 2021, 46 (24) : 6055 - 6058
  • [5] Chronic monitoring of cortical hemodynamics in behaving, freely-moving rats using a miniaturized head-mounted optical microscope
    Sigal, Iliya
    Gad, Raanan
    Koletar, Margaret
    Ringuette, Dene
    Stefanovic, Bojana
    Levi, Ofer
    [J]. CLINICAL AND TRANSLATIONAL NEUROPHOTONICS; NEURAL IMAGING AND SENSING; AND OPTOGENETICS AND OPTICAL MANIPULATION, 2016, 9690
  • [6] A miniature head-mounted two-photon microscope: High-resolution brain imaging in freely moving animals
    Helmchen, F
    Fee, MS
    Tank, DW
    Denk, W
    [J]. NEURON, 2001, 31 (06) : 903 - 912
  • [7] A HEAD-MOUNTED MICROMANIPULATOR FOR RECORDING UNIT-ACTIVITY IN FREELY-MOVING RATS
    ZALCIK, J
    SVORAD, D
    [J]. PHYSIOLOGIA BOHEMOSLOVACA, 1980, 29 (03): : 287 - 287
  • [8] Three-photon imaging of synthetic dyes in deep layers of the neocortex
    Chao J. Liu
    Arani Roy
    Anthony A. Simons
    Deano M. Farinella
    Prakash Kara
    [J]. Scientific Reports, 10
  • [9] Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice
    Mathew L. Rynes
    Daniel A. Surinach
    Samantha Linn
    Michael Laroque
    Vijay Rajendran
    Judith Dominguez
    Orestes Hadjistamoulou
    Zahra S. Navabi
    Leila Ghanbari
    Gregory W. Johnson
    Mojtaba Nazari
    Majid H. Mohajerani
    Suhasa B. Kodandaramaiah
    [J]. Nature Methods, 2021, 18 : 417 - 425
  • [10] Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice
    Rynes, Mathew L.
    Surinach, Daniel A.
    Linn, Samantha
    Laroque, Michael
    Rajendran, Vijay
    Dominguez, Judith
    Hadjistamoulou, Orestes
    Navabi, Zahra S.
    Ghanbari, Leila
    Johnson, Gregory W.
    Nazari, Mojtaba
    Mohajerani, Majid H.
    Kodandaramaiah, Suhasa B.
    [J]. NATURE METHODS, 2021, 18 (04) : 417 - +
12345