The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen

被引：0

作者：

I. Yu. Shchit

K. B. Ignatov

T. Yu. Kudryavtseva

N. A. Shishkova

R. I. Mironova

L. I. Marinin

A. N. Mokrievich

V. M. Kramarov

S. F. Biketov

I. A. Dyatlov

机构：

[1] Federal Service for the Protection of Customer Rights,State Research Center of Applied Microbiology and Biotechnology

[2] Russian Academy of Sciences,Vavilov Institute of General Genetics

[3] Russian Academy of Sciences,All

来源：

Molecular Genetics, Microbiology and Virology | 2017年 / 32卷

关键词：

loop isothermal amplification of DNA; LAMP;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The results of detection and identification of Bacillus anthracis strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasmid DNA targets specific for B. anthracis strains has been demonstrated. No cross reactions with DNA from bacterial strains of other species of the B. cereus group were detected. The development of tests for anthrax-pathogen detection based on the optimized reaction of loop isothermal DNA amplification is planned. These tests will be convenient for clinical studies and field diagnostics due to the absence of requirements for sophisticated equipment.

引用

页码：100 / 108

页数：8

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