The complete mitochondrial genome of the mixtured pygmy frog Microhyla mixtura (Anura, Microhylidae)

The mixtured pygmy frog (Microhyla mixtura, Liu and Hu 1966) is an endemic species in China, which has suffered habitat degradation and isolation recently. In the present study, the complete mitochondrial genome of M. mixtura was sequenced and annotated. The 16,729-bp circular mitochondrial genome contained a conserved set of 37 mitochondrial genes including 13 protein-coding genes (PCGs), 22 transfer RNA, 2 ribosomal RNA genes, and a control region. The gene order was identical to the typical vertebrate gene arrangement. The overall base composition of this genome was as follows: 29.5% A, 24.9% C, 31.4% T, 14.2% G, with a total A+T content of 60.9%. Both phylogenetic trees based on the maximum likelihood and Bayesian inference showed that Microhyla was grouped within Microhylinae and M. mixtura was closely related to Microhyla okinavensis.