Expression of Recombinant Proteins in Pichia Pastoris

被引：0

作者：

Pingzuo Li

Anukanth Anumanthan

Xiu-Gong Gao

Kuppusamy Ilangovan

Vincent V. Suzara

Nejat Düzgüneş

V. Renugopalakrishnan

机构：

[1] Chinese Academy of Sciences,Shanghai Research Center of Biotechnology

[2] Harvard Medical School,Children’s Hospital

[3] Harvard Medical School,Department of Biological Chemistry and Molecular Pharmacology

[4] National Cancer Institute,Laboratory of Cell Biology

[5] National Institutes of Health,Centro de Investigacion en Calidad Ambiental

[6] Tecnologico de Monterrey,Department of Microbiology, Arthur A. Dugoni School of Dentistry

[7] Campus Estado de México,Bionanotechnology Group

[8] University of the Pacific,Biophotovoltaic Group, Division of Bioengineering and Department of Mechanical Engineering, NUS Nanotechnology Initiative (NUSNNI)

[9] Florida International University,undefined

[10] National University of Singapore,undefined

来源：

Applied Biochemistry and Biotechnology | 2007年 / 142卷

关键词：

Protein expression; Methanol induction; Dissolved oxygen; Gene integration; Alcohol oxidase promoter; AOX1;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.

引用

页码：105 / 124

页数：19

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