Time-resolved fluoroimmunoassay

被引：0
|
作者：
Paolo Degan
Adriano Podestà
Giorgio Montagnoli
机构：
[1] National Institute for Research on Cancer,Department of Anatomy, Biochemistry and veterinary Physiology
[2] University of Pisa,undefined
来源：
Molecular Biotechnology | 1999年 / 13卷
关键词：
Fluoroimmunoassay, time-resolution; europium ion; flourescence enhancement;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A method is reported to set up a standard competitive TR-FIA. A simple and inexpensive way to prepare reagents and carry out operations is presented as well, with the aim to make it possible to perform a very sensitive analytical procedure in a personalized way within a nondedicated biochemistry laboratory. This protocol is general and can be easily modified with consideration to the analytical target. Once the antibody is available, both its labeling with diethylenetriaminepentaacetic acid dianhydride and Eu3+, and the setting-up of the assay with measurement of europium ion time-resolved fluorescence in a home-made enhancement solution become feasible.
引用
收藏
页码：215 / 222
页数：7
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