Synthetic silica fibers of different length, diameter and shape: synthesis and interaction with rat (NR8383) and human (THP-1) macrophages in vitro, including chemotaxis and gene expression profile

被引：0

作者：

Bialas, Nataniel ^{[1
,2
]}

Rosenkranz, Nina ^{[3
]}

Weber, Daniel Gilbert ^{[3
]}

Kostka, Kathrin ^{[1
,2
]}

Johnen, Georg ^{[3
]}

Winter, Aileen ^{[1
,2
]}

Brik, Alexander ^{[3
]}

Loza, Kateryna ^{[1
,2
]}

Szafranski, Katja ^{[3
]}

Bruening, Thomas ^{[3
]}

Buenger, Juergen ^{[3
]}

Westphal, Goetz ^{[3
]}

Epple, Matthias ^{[1
,2
]}

机构：

[1] Univ Duisburg Essen, Inorgan Chem, D-45141 Essen, Germany

[2] Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CENIDE, D-45141 Essen, Germany

[3] Inst Ruhr Univ Bochum IPA, Inst Prevent & Occupat Med, German Social Accid Insurance, D-44789 Bochum, Germany

来源：

PARTICLE AND FIBRE TOXICOLOGY | 2024年 / 21卷 / 01期

关键词：

PULMONARY TOXICITY; CARBON NANOTUBES; ASBESTOS; NANOPARTICLES; NANOMATERIALS; EXPOSURE; BURDEN; INJURY;

D O I：

10.1186/s12989-024-00586-6

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Background Inhalation of biopersistent fibers like asbestos can cause strong chronic inflammatory effects, often resulting in fibrosis or even cancer. The interplay between fiber shape, fiber size and the resulting biological effects is still poorly understood due to the lack of reference materials.Results We investigated how length, diameter, aspect ratio, and shape of synthetic silica fibers influence inflammatory effects at doses up to 250 mu g cm-2. Silica nanofibers were prepared with different diameter and shape. Straight (length ca. 6 to 8 mu m, thickness ca. 0.25 to 0.35 mu m, aspect ratio ca. 17:1 to 32:1) and curly fibers (length ca. 9 mu m, thickness ca. 0.13 mu m, radius of curvature ca. 0.5 mu m, aspect ratio ca. 70:1) were dispersed in water with no apparent change in the fiber shape during up to 28 days. Upon immersion in aqueous saline (DPBS), the fibers released about 5 wt% silica after 7 days irrespectively of their shape. The uptake of the fibers by macrophages (human THP-1 and rat NR8383) was studied by scanning electron microscopy and confocal laser scanning microscopy. Some fibers were completely taken up whereas others were only partially internalized, leading to visual damage of the cell wall. The biological effects were assessed by determining cell toxicity, particle-induced chemotaxis, and the induction of gene expression of inflammatory mediators.Conclusions Straight fibers were only slightly cytotoxic and caused weak cell migration, regardless of their thickness, while the curly fibers were more toxic and caused significantly stronger chemotaxis. Curly fibers also had the strongest effect on the expression of cytokines and chemokines. This may be due to the different aspect ratio or its twisted shape.

引用

页数：20