Pavlovian conditioning of the proboscis extension reflex in harnessed foragers using paired vs. unpaired and discrimination learning paradigms: tests for differences among honeybee subspecies in TurkeyConditionnement pavlovien du réflexe d’extension du proboscis chez des butineuses en contention à l’aide de paradigmes appariés et non appariés pour l’apprentissage discriminatoire: tests de différences parmi les sous-espèces d’abeilles domestiques en TurquiePawlowsche Konditionierung des Rüsselreflexes bei fixierten Sammlerinnen mit verbundenen und unverbundenen Ansätzen für diskriminatorisches Lernen: Test auf Unterschiede zwischen Bienenrassen in der Türkei

Experiments utilized three honeybee subspecies from very distinct biomes (Apis mellifera caucasica, A.m. carnica, A.m. syriaca). In experiment one a simple association between odor and a sucrose feeding was readily established in all three subspecies. This association decreased when the conditioned stimulus was no longer followed by a feeding. Neither the learning rate nor extinction rate differed among subspecies. Unpaired controls confirmed that the acquisition of the odor-food association is learned. In experiment two, an attempt to uncover subspecies differences was tested through the ability of bees to discriminate between two odors, one of which is paired with a feeding. Rapid learning occurred in all subspecies and no significant subspecies differences were observed. Finally, discrimination learning was used as an added control to test for honeybee response to an olfactory versus mechanical (air) stimulus.